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KMID : 1024420130170010024
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Food Engineering Progress
2013 Volume.17 No. 1 p.24 ~ p.32
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Molecular Properties of Polysaccharide Rich Fraction from Ultra-filtration of Aloe vera Gel
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Kwon Hye-Mi
Beak Jin-Hong
Cha Ji-Min
Lee Shin-Young
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Abstract
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Two fractions (permeate and retentate) from fiber-free Aloe vera gel were separated using ultra-filtration (UF) system with tubular ceramic membrane (MWCO of 50 kDa), and their molecular properties were investigated. The retentate of UF Aloe gel was a polysaccharide-rich fraction containing about 2.3-fold higher polysaccharide content than fiber-free gel. The FT-IR and 1H NMR spectra of this fraction showed the characteristic patterns of β-binding polysaccharide and a higher degree of acetylation indicating a higher level of bioactive polysaccharide content. The
molecular weight and polydispersity of polysaccharide fraction from GPC (gel permeation chromatography) were determined to be 36.8-43.9 kDa and 1.24, respectively, indicating a kind of undispersed polysaccharide. From the SEM observation, the surface structure of polysaccharide fraction had a gel cluster-like structure with a convoluted rough surface. The molecular conformation by Congo red assay exhibited a property of helix structure confirming the existence of a higher-ordered structure as a biological activity conformation.
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KEYWORD
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Aloe vera gel, ultra-filtration fraction, polysaccharide, molecular property
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